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Combined Trichinella Spiralis Antibody Rapid Test (310300)

Combined Trichinella Spiralis Antibody Rapid Test (310300)
  • Product Detials:Catalog No. T. S-01 For Professional Use Only Summary Trichinella spiralis can lead to Trichinella spiralisis, and then the specific antibody against Trichinella spiralis arise in human body, hereby detecting the specific antibody against Trichinella spiralis can be the secondary diadynamic criteria. Reagents and contents 1 8X Coated microtiter strips: Ready to use 2 1X Conjugate solution: Ready to use 3 1X Washing solution: Ready to use 4 1X Substrate A solution: Ready to use 5 1X Substrate B solution: Ready to use 6 1X Sample dilution: Ready to use 7 1X Stopping solution: Ready to use Warning: Stopping solution irritates eyes and skin. Keep out of the reach of children. Upon contact with the eyes, rinse thoroughly with water and consult a doctor. 8 1X Positive control: Ready to use 9 1X Negative control: Ready to use 10 1X Instruction sheet Test procedure 1 Sample preparation: Dilutes the patient' s serum with sample dilution at 1: 50, e. G. 10ul serum + 0.5 ml sample dilution, and mix thoroughly. 2 Strip wells preparation: Dispense a drop of washing solution to each well, discard the fluid by tapping the strips on tissue paper. 3 Adding samples and controls Leave well A1 for reagent blank. Pipette controls and samples as follows: 100ul of negative control and positive control respectively, and 100 ul diluted samples each into remaining wells. Incubation at 37degree centigradefor 15 minutes. Discard off contents of the wells and add each well of washing solution, fill all wells with distilled water completely, incubate for 15 seconds and discard off. Perform another 4 washing cycles as above. At the end of the washing step carefully remove remaining fluid by tapping the strips on the tissue paper prior to the next step. 4 Adding conjugate solution Dispense 2 drops into all wells and incubate at 37degree centigrade for 15 minutes. Discard the contents of the wells and wash 5 times as described in step 3. 5 Adding substrates Dispense one drop of substrate A solution and B solution respectively, incubate at room temperature (37degree centigrade)for 10 minutes. Dispense one drop of stopping solution into all wells. Zero the ELISA microtiter plate reader using the reagent blank well A1. Measure the absorbance at 450 nm. Results 1 Observation with naked eyes before adding stopping solution Negative: No apparent blue appears in the sample wells. This indicates that no cysticercus antibody has been detected. Positive: Apparent blue appears in the sample wells. This indicates that the sample contains cysticercus antibody. Invalid: If no blue appears in the positive control well or apparent blue is observed in the negative control well, this indicates a possible error in performing the test. The test should be repeated using a new kit. 2 Judgment by microtiter plate reader Cut-off value = 2.1XA- (absorbance of negative control) Interpretation of results A450 (patient) ≥ cut-off: Anti- Trichinella spiralis antibody positive A450 (patient) < cut-off: Anti- Trichinella spiralis antibody negative Notes 1 store at 2-8, make the temperature of the kit return to room temperature before use. Screw down the cap after use, don't mix caps between diffirent bottles and don't mix components between diffirent kits 2 fill wells with distilled water when washing, 30 seconds each time, pour out the liquid. Prohibit to use tap water or any other water. 3 recommand to judge results with instruments to improve comparability of testing. More Product Features Trademark: COMBINED Model NO.: 310300 Standard: ISO9001:2000 Company: Shenzhen Combined Biotech Co., Ltd.
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Contact Info
Company： Shenzhen Combined Biotech Co.,Ltd
Area： China/Guangdong
Contact： Mr. LI Hailong
Tel： 86-755-26650513
Fax： 86-755-26650520
Web site：
Add： Unit 2d, Building 8th, Tian-An Industrial Park, Nanyou, Nanshan District, Shenzhen, Guangdong, China
Zip： 518054

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   Shenzhen Combined Biotech Co.,Ltd

     Founded in 2003, Shenzhen Combined Biotech Co., Ltd (BIOCBD) is a high-tech enterprise, aiming ..... more >>

Contact： Mr. LI Hailong 

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