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Home > Products > Combined Spirometra Mansomi Antibody Rapid Test (311100)
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Combined Spirometra Mansomi Antibody Rapid Test (311100)

Combined Spirometra Mansomi Antibody Rapid Test (311100)
  • Product Detials:Combined Spirometra mansomi Antibody Rapid Test Catalog No. S. J-01 For Professional Use Only Summary The imago and Spirometra mansomi, hereby detecting the specific antibody against Spirometra mansomi can be the secondary diadynamic criteria. The test take the Spirometra mansomi eggs antigen as the solid phase antigen to detect specific antibody against Spirometra mansomi in serum. Reagents and contents 1 8X Coated microtiter strips: Ready to use 2 1X Conjugate solution: Ready to use 3 1X Washing solution: Ready to use 4 1X Substrate A solution: Ready to use 5 1X Substrate B solution: Ready to use 6 1X Sample dilution: Ready to use 7 Stopping solution: Ready to use Warning: Stopping solution irritates eyes and skin. Keep out of the reach of children. Upon contact with the eyes, rinse thoroughly with water and consult a doctor. 8 1X Positive control: Ready to use 9 1X Negative control: Ready to use 10 1X Instruction sheet Test procedure 1 Sample preparation: Dilute the patient' s serum with sample dilution at 1: 100, e. G. 10 ul serum + 1 ml sample dilution, and mix thoroughly. 2 Adding samples and controls Leave well A1 for reagent blank. Pipette controls and samples as follows: 100ul negative control and positive control respectively, and 100ul diluted samples each into remaining wells. Incubation at 37 degree centigrade for 15 minutes. Discard off contents of the wells and add each well of washing solution, fill all wells with distilled water completely, incubate for 15 seconds and discard off. Perform another 4 washing cycles as above. At the end of the washing step carefully remove remaining fluid by tapping the strips on the tissue paper prior to the next step. 3 Adding conjugate solution Dispense 2 drops into all wells and incubate at 37 degree centigrade for 15 minutes. Discard the contents of the wells and wash 5 times as described in step 2. 4 Adding substrates Dispense one drop of substrate A solution and B solution respectively, incubate at room temperature (37 degree centigrade)for 10 minutes. Dispense one drop of stopping solution into all wells. Zero the ELISA microtiter plate reader using the reagent blank well A1. Measure the absorbance at 450 nm. Results 1 Observation with naked eyes before adding stopping solution Negative: No apparent blue appears in the sample wells. This indicates that no cysticercus antibody has been detected. Positive: Apparent blue appears in the sample wells. This indicates that the sample contains cysticercus antibody. Invalid: If no blue appears in the positive control well or apparent blue is observed in the negative control well, this indicates a possible error in performing the test. The test should be repeated using a new kit. 2 Judgment by microtiter plate reader Cut-off value = 2.1XA- (absorbance of negative control) Interpretation of results A450 (patient) ≥ cut-off: Anti- Spirometra mansomi antibody positive A450 (patient) < cut-off: Anti- Spirometra mansomi m antibody negative Notes 1 Always brings all reagents, samples and controls to room temperature before use. 2 Don' t use tap water for washing steps. 3 Recommend to judge result by microtiter plate reader and to set duplicate negative control to reduce deviation More Product Features Trademark: COMBINED Model NO.: 311100 Standard: ISO9001:2000 Company: Shenzhen Combined Biotech Co., Ltd.
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Contact Info
Company： Shenzhen Combined Biotech Co.,Ltd
Area： China/Guangdong
Contact： Mr. LI Hailong
Tel： 86-755-26650513
Fax： 86-755-26650520
Web site：
Add： Unit 2d, Building 8th, Tian-An Industrial Park, Nanyou, Nanshan District, Shenzhen, Guangdong, China
Zip： 518054

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   Shenzhen Combined Biotech Co.,Ltd

     Founded in 2003, Shenzhen Combined Biotech Co., Ltd (BIOCBD) is a high-tech enterprise, aiming ..... more >>

Contact： Mr. LI Hailong 

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