| Description du produit: |
Assay principle
The T3 EIA, a second antibody (goat anti-mouse IgG) is coated on a microtiter wells. A measured amount of patient
Serum, a certain amount of mouse monoclonal Anti-T3 antibody, and a constant amount of T3 conjugated with horseradish peroxidase are added to the microtiter wells. During incubation, the mouse anti-T3 antibody is bound to the second antibody on the wells, T3 and the enzyme conjugated-T3 compete for the limited binding sites on the anti-T3 antibody. After a 60 minute incubation at the temperature 37, the wells are washed 5 times by water to remove unbound T3 conjugate. A solution of H2O2/TMB is then added and incubated for 20 minutes at room temperature, resulting in the development of a blue color. The color development is stopped with the addition of 1N HCl, and the absorbance is measured spectrophotometrically at 450 nm. The intensity of the color formed is proportional to the amount of enzyme present, and is inversely related to the amount of unlabeled T3 standards assayed in the same way. The concentration of T3 in the unknown sample is then calculated. 7-11
Trademark:
JD
Model NO.:
JT003
Company:
Jei Daniel Biotech Corp. |
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